USPTO Patents Application 09828615

1/20

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2/20

FIGURE 2

INHIBITOR

CONCENTRATION

ASSAY

COMBINATION INDEX

CiJIVliJlrl A 1 ivIPi

90% Inhibition

50V. Inhibition

PAI2:2D7

10:1

Entry

0.043

0.291

Z. 1

riision

0,017

A A1 n

o.oiy

10*1

rusion

50:1

Fusion

0.158

0.046

PA12:PA14

10:1

Entry

0.437

0.535

10:1

Fusion

0.22

0.263

PA14:2D7

1:1

Entry

2.85

1.85

1:1

Fusion

1.34

1.74

PA12:PA11

1:1

Entry

0.707

0.641

PA12:RANTES

1000:1

Fusion

0.331

0.156

PA14:RANTES

100:1

Fusion

1.6

1.37

2D7:RANTES

100:1

Fusion

0.972

0.962

PA12:CD4-IgG2

10:1

Fusion

0.3

0.31

PA14:CD4.IgG2

1:1

Fusion

0.957

0.566

2D7:CD4-IgG2

1:1

Fusion

1.127

0.302

3/20

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4/20

FIGURE 4

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2D7

PAS

PA9
PA10
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PA12
PA14

2D7
PAS
PAS
^ PA10

f*l PA11 tX

PA 12
PA14

2D7

PAS
PA9
^ PAID

170

175

180

185

190

PA 14

2D7
PAS
2 PA9
U PA10
PA11
PA12
PA 14

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265

270

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51%-75%
16%-50%
0%-25V.
nd

1

5/20

FIGURE 5A

50 too 150 200 250 300 350 400

Time (seconds)

6/20

FIGURE SB

7/20

FIGUKE6A

8/20

BlGXJItE 6B

9/20

FIGURE 6C

1400 1

400

Concentration (nM)

-♦-b-JRFL:sCD4
-»-b-sCD4 alone
-*-b-JRFL alone
->«-b-LAI:sCD4

10/20

FIGURE 6D

11/20

12/20

13/20

FIGURE 9

Combination Index
CD4-IgG2:T-20 Mass Ratio
Percent 25:1 25:1

Inhibition

(low)

(high)

5:1

1:1

95

0.32

0.20

0.22

0.50

90

0.38

0.25

0.27

0.55

85

0.43

0.29

0.30

0.59

80

0.47

0.33

0.34

0.62

75

0.51

0.36

0.37

0.65

70

0.54

0.39

0.40

0.67

65

0.58

0.42

0.43

0.70

60

0.61

0.45

0.45

0.73

55

0.65

0.48

0.49

0.75

50

0.69

0.51

0.52

0.78

14/20

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